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Aspergillus niger library (LMBP LIB8)
| Library name: | Aspergillus niger library |
| Library type: | cDNA |
| Library accession number: | LMBP LIB8 |
| Organism: | Aspergillus niger |
| Priming method: | NotI-(dT)15 (unidirectional) |
| Vector name: | pSCGAL10-SN |
| Vector sequence file: | p2471.txt |
| Vector circular map: | p2471.pdf |
| Vector accession number: | LMBP 2471 |
| Vector type: | yeast expression vector |
| Cloning sites: | SfiI-NotI |
| Library coverage: | 4,0 x 10E5 |
| Average insert size: | 1,25 kb |
| Distributed as: | pooled library DNA |
| DNA concentration: | 200 ng/µl |
| Volume: | 5 µl |
| Further information: | Aspergillus niger N402 was grown in rich YPD medium, consisting of 1% bacto-yeast extract, 2% bacto-peptone and 2% dextrose. The host strain Escherichia coli K12 MC1061 or DH5α can be used for electroporation to amplify the library. The transformation mixture can then be streaked on selective solid medium (LB-Lennox + 100 µg/ml ampicillin) and cultivated at 37°C to obtain the different library clones. |
| History of deposit: | The cDNA library was deposited by Prof. R. Contreras1. 1 Department of Molecular Biology, Ghent University, Belgium |
| Library reference: | Veldhuisen et al., Mol. Gen. Genet. 256 (1997), 446-455 [PMID: 9393442] |
| Related reference: | - |
| Restricted distribution: | - BCCM MTA |
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